NRAMP5 (NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 5) : Os07g0257200

RAP-DB

Locus ID: Os07g0257200
Transcript ID: Os07t0257200-01 (chr07:8871643-8878905)
Description: Manganese and Cadmium transporter, Mn and Cd uptake
RAP-DB Gene symbol: NRAMP5, OsNRAMP5, OsNramp5
RAP-DB Gene name: NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 5, BACTERIOCIDE EFFECT 5
Gene Ontolgy: transporter activity (GO:0005215)
transport (GO:0006810)
membrane (GO:0016020)
Links: TASUKE (for RAP-DB), GBrowse

Oryzabase

CGSNL Gene symbol: NRAMP5
CGSNL Gene name: NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 5
Oryzabase Gene symbol: OsNRAMP5, OsNramp5
Oryzabase Gene name: BACTERIOCIDE EFFECT 5
Gene Ontolgy: transporter activity (GO:0005215)
transport (GO:0006810)
ion transport (GO:0006811)
manganese ion transport (GO:0006828)
cadmium ion transport (GO:0015691)
membrane (GO:0016020)
integral to membrane (GO:0016021)
metal ion transport (GO:0030001)
response to cadmium ion (GO:0046686)
iron ion homeostasis (GO:0055072)
Trait Ontolgy: -
Plant Ontolgy: -
Links: Oryzabase

Gene information from literatures

Gene function

  • OsNRAMP5, which encodes a natural resistance-associated macrophage protein, contributes to Mn, Cd, and Fe transport in rice and is important for plant growth and development. Functional analysis revealed that the defective transporter protein encoded by the mutant osnramp5 greatly decreases Cd uptake by roots, resulting in decreased Cd in the straw and grain.

Functional variations

Representative variations
#Var Cultivar 1 Cultivar 2 Positions (protein or transcript) Description References
V1 NPB, Koshihikari lcd-kmt1 (osnramp5-1)1 g.8875087_8875118delins[mPingA1 sequence (433 bp)] A 433-bp insertion was observed in the exon X of lcd-kmt1 (32 bp in exon X of the WT with 50 bp in lcd-kmt1 and remaining 383 bp of the insertion in intron X). The inserted sequence was identical to mPingA1. No significant negative effects on plant or grain morphology, eating quality, grain or straw yield, indicating that lcd-kmt1 can be used directly in breeding programs. PMID:23132948
V2 NPB, Koshihikari lcd-kmt2 (osnramp5-2)1 g.8875509delG
p.Thr306fs*43
A single-nucleotide deletion was observed in exon IX of lcd-kmt2. No significant negative effects on plant or grain morphology, eating quality, grain or straw yield, indicating that lcd-kmt2 can be used directly in breeding programs. PMID:23132948
V3 NPB, Koshihikari lcd-kmt3 (osnramp5-3)2 An ∼227-kb deletion including all of OsNRAMP5 was observed in lcd-kmt3. lcd-kmt3 showed earlier heading and smaller plant size compared to WT, presumably because of the large DNA deletions in this mutant. PMID:23132948
V4 NPB, Zhonghua 11 osnramp5 mutant A T-DNA insertion was observed in the 5th intron of the OsNRAMP5 mutant. The knockout mutant of OsNRAMP5 increased the sensitivity to Mn and Fe deficiency. Cd in leaves was significantly lower in the mutant compared with WT. PMID:24963001
V5 NPB, Zhonghua 11 osnramp5 mutan A T-DNA insertion was observed in the 12th intron of the OsNRAMP5 mutant. The mutant showed huge reductions of Cd and Mn accumulation, but the grain yield of this mutant was only 11% of WT. The leaves of the knockout mutant showed severe chlorotic symptoms. PMID:22589467

1Accelerated carbon ion-beam mutagenesis mutant from Koshihikari (Low-Cd mutant). There was no difference in plant growth among WT, lcd-kmt1 and lcd-kmt2, 2Accelerated carbon ion-beam mutagenesis mutant from Koshihikari (Low-Cd mutant). The growth of lcd-kmt3 was reduced under the sufficient Mn level in hydroponics.

Gene expression

  • Microarray analysis showed a 2.5-fold increase in OsNRAMP5 expression for lcd-kmt1 compared with the WT. Genes involved in the photosynthetic process were up-regulated considerably, and Fe-deficiency inducible genes were down-regulated in the lcd-kmt1 mutant (PMID:23132948).
  • OsNRAMP5 expression was restricted to roots epidermis, exodermis, and outer layers of the cortex as well as in tissues around the xylem. OsNRAMP5 localized to the plasma membrane (PMID:22368778).
  • The expression of OsNRAMP5 is not controlled by Fe deficiency in root and was also observed in pistil, ovary, lemma and palea (PMID:22751306).
  • OsNRAMP5 was expressed with a strong tissue-specific pattern. OsNRAMP5 exhibited the strongest expression signal in young reproductive tissues, e.g. panicle, spikelet, and hull. OsNRAMP5 also showed high expression levels in plumules and young shoots, leaves and roots, and a moderate level in stems at the heading stage. On the other hand, little or no expression of OsNRAMP5 was observed in the mature leaf, flag leaf, leaf sheath, or endosperm (PMID:24963001).
  • OsNRAMP5 was highly expressed in the stele and sclerenchyma layer of roots, unexpanded leaves, culms, and the border region of nodes, but only moderately expressed in expanded leaves. There was little expression in the mature leaf sheaths. Furthermore, high-magnification observation showed an enrichment of OsNRAMP5 expression in the vascular bundles, especially in the parenchyma cells surrounding the xylem (PMID:24963001).
  • The expression of a Mn transporter gene, OsNramp5, was not affected by a short exposure (<1 day) to Silicon (Si), but down-regulated by relatively long-term exposure to Si in WT. In contrast, the expression of OsNramp5 was unaffected by Si in the lsi1 (low silicon rice 1) mutant (PMID:26733690).

Other information

  • An nramp5 knockout mutant and RNAi lines grown in soil showed a strongly reduced Cd concentration in the straw and the grain, corresponding with a much lower short-term uptake rate of rice roots for Cd. PMID:22981394
  • OsNRAMP1 does not contribute to Mn uptake. OsNRAMP5 plays an important role in the translocation and distribution of Mn in rice plants (PMID:22368778).
  • Knockout of OsNRAMP5 increased the sensitivity to Mn and Fe deficiency. OsNRAMP5 not only contributes to Mn uptake but also to the root-to-shoot translocation of Mn. Uptake of Fe and Cd mediated by OsNRAMP5 was affected by Mn concentration. Mn and Cd showed different distributions in leaves and were affected by the absence of OsNRAMP5. Cd in leaves was significantly lower in osnramp5 plants compared with WT. OsNRAMP5 contributed to Mn accumulation in hulls and brown rice (PMID:24963001).
  • The development of new indica rice lines with low Cd accumulation and no transgenes by knocking out the metal transporter gene OsNramp5 using CRISPR/Cas9 system. Hydroponic culture showed that Cd concentrations in shoots and roots of osnramp5 mutants were dramatically decreased in high Cd condition. The plant yield was not significantly affected in osnramp5 mutants. A practical approach to developing Cd pollution-safe indica rice cultivars that minimizes Cd contamination risk in grains has been proposed (PMID:29089547).

References

  • Ishikawa S et al. 2012 Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice. /Proc Natl Acad Sci U S A. PMID:23132948
  • Sasaki A et al. 2012 Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice. Plant Cell PMID:22589467
  • Ishimaru Y et al. 2012 Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport. Sci Rep. PMID:22368778
  • Ishimaru Y et al. 2012 OsNRAMP5, a major player for constitutive iron and manganese uptake in rice. Plant Signal Behav. PMID:22751306
  • Clemens S et al. 2013 Plant science: the key to preventing slow cadmium poisoning. Trends Plant Sci. PMID:22981394
  • Yang M et al. 2014 OsNRAMP5 contributes to manganese translocation and distribution in rice shoots. J Exp Bot. PMID:24963001
  • Che J et al. 2016 Silicon decreases both uptake and root-to-shoot translocation of manganese in rice. J Exp Bot. PMID:26733690
  • Tang L et al. 2017 Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield. Sci Rep. PMID:29089547