Inconsistency between cDNA and genomic sequences


The cDNA should be a copy of (part of) the genomic DNA, but their sequences are not always identical for several reasons such as sequencing errors and polymorphisms. The cDNA sequences are in general error-prone because of experimental artefacts. Therefore, when a cDNA sequence is different from a corresponding genomic sequence, we decided to use the genomic one. Even though a RAP locus was identified by a full-length cDNA, the nucleotide sequence displayed in the RAP-DB is not necessarily identical to that of the cDNA.